Microscopy
Method
- Place onion cell on slide, add a drop of iodine stain.
- Lower coverslip with a mounted needle at 45° to avoid air bubbles.
- Start on the lowest objective lens, focus with coarse then fine knob.
- Move to higher magnification once focused.
- Draw with sharp pencil, clear continuous lines, no shading, label with ruler.
Calculations
Magnification = image size ÷ real size. Always convert units (1 mm = 1000 μm).
Why iodine?
Stains starch granules so structures show up clearly under the microscope.